Isolation and Characterization of the lacA Gene Encoding b-Galactosidase in Bacillus subtilis and a Regulator Gene, lacR
نویسندگان
چکیده
منابع مشابه
Isolation and characterization of the lacA gene encoding beta-galactosidase in Bacillus subtilis and a regulator gene, lacR.
We have isolated transposon insertions in the lacA gene encoding an endogenous beta-galactosidase of Bacillus subtilis. Upstream of the putative operon containing lacA is a negative regulator, lacR, which encodes a product related to a family of regulators that includes the lactose repressor, lacI, of Escherichia coli. New strains with insertions in the lacA gene should be of use in studies usi...
متن کاملCloning, High-level Expression and Characterization of a β-Galactosidase from Bacillus subtilis G1
A gene (lacA) coding for β-galactosidase from Bacillus subtilis strain G1 was cloned, expressed in E. coli and the β-galactosidase LacA was purified and characterized. The lacA gene consisted of an open reading frame of 2061 nucleotides, encoding a 686-aa protein, with a predicted molecular mass of 79 kDa and pI 6.16. The β-galactosidase showed an identity of 97.8% with the corresponding amino ...
متن کاملCloning of the Gene Encoding M2e of Influenza Virus in B. subtilis
Background and Aims: The ectodomain of matrix protein of influenza virus is a weak immunogen that is highly conserved among all subtypes of influenza A virus. Tandem repeats of these genes along with linker were used to enhance immunogenicity of M2e protein and so it can be served as a universal vaccine in both humans and livestock. Materials and Methods: In this study, the sequences of extra-d...
متن کاملResearch Article: Isolation, biochemical and molecular detection of Bacillus subtilis and Bacillus licheniformis from the digestive system of rainbow trout (Oncorhynchus mykiss) and its inhibitory effect on Aeromonas hydrophila
Our study was focused on characterization of dominant Bacillus bacteria isolated from the digestive tract of Oncorhynchus mykiss. After 8 weeks, the fish fed with probiotic-free diet were dissected under strictly controlled conditions. Fish intestines were eviscerated and its content diluted to 10−4 using Ringer solution. Two isolated bacteria were Gram-positive, catalase-positive, and oxidase-...
متن کاملCloning and Enhanced Expression of an Extracellular Alkaline Protease from a Soil Isolate of Bacillus clausii in Bacillus subtilis
in the detergent industry. In this study, the extracellular alkaline serine protease gene, aprE, from Bacillusclausii was amplified by PCR and further cloned and expressed in B. subtilis WB600 using the pWB980 expression vector. Protease activity of the recombinant B. subtilis WB600 harboring the plasmid pWB980/aprEreached up to 1020 U/ml, approximately 3-folds higher than the nativ...
متن کامل